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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been observed in several tumors, which leads to chromosome instability, and thus could lead to tumorigenesis. This protein was located to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a vital role in endorsing mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, happen to be described. Two added splice variants were reported but thorough nucleotide sequence information and facts isn't nevertheless out there.
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Blocking peptides are peptides that bind precisely into the concentrate on antibody and block antibody binding. These peptide commonly has the epitope acknowledged via the antibody.
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This information outlines further more feasible optimization of cellular and immunoassay parameters to make sure the best possible results are...
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This tutorial outlines more probable optimization of mobile and immunoassay parameters to be sure the very best outcomes are...
Antibodies sure to the blocking peptide not bind into the epitope over the concentrate on protein. By evaluating the staining in the blocked antibody versus the antibody by yourself, you can see which staining is precise.
Goal Info Cyclin E belongs to the highly conserved cyclin family members, whose members are characterised by a extraordinary periodicity in protein abundance from the mobile cycle. Cyclins perform as regulators of CDK kinases. Distinct cyclins show distinctive expression and degradation patterns which add on the temporal coordination of each mitotic event. Cyclin E sorts a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S transition. This protein accumulates in the G1-S period boundary and is also degraded as cells development by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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